Composite

Part:BBa_K3257117:Design

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-13)


T7 Promoter-lox5171-R-PPT-RBS-ChlR_K182X-PBS-U5-R-loxP-T7 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1107
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 945
    Illegal BsaI.rc site found at 966


Design Notes

The lox sites, PBS and gene of interest should be changeable according to the users' demands and purposes.


Source

Composite from the registry.

References